14-3-3 Recombinant Mouse Monoclonal Antibody

SKU: orb1172936

Description

14-3-3 Recombinant Mouse Monoclonal Antibody

Research Area

Alzheimer's Disease, Cancer Research, Cell Biology, Cytoplasmic, Neurology, Neuroscience, Signal Transduction, Synapse Markers, Tumor Biology

Images & Validation

• Tested Applications: ICC, WB
• Dilution Range: WB=1:500-2000, ICC/IF=1:50-200
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: IgG1, Kappa
• Clone No.: BD34E3
• Target: YWHAZ
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GW128; HEL-S-1; HS1; KCIP-1; YWHAA; 14-3-3-zeta; HEL-S-3; HEL-S-93; HEL4; POPCHAS; YWHAD; 1110013I11Rik; 14-3-3zeta; 1300003C17Rik; 14-3-3b; 14-3-3z; 143B_BOVIN; 1433B_HUMAN; YWHAB; Protein 1054; Protein kinase C inhibitor protein 1 (KCIP-1); 1433Z_HUMAN; YWHAZ; 143Z_MOUSE; 1433B_MOUSE; 1433B_RAT; Prepronerve growth factor RNH-1; 1433Z_RAT; Mitochondrial import stimulation factor S1 subunit; Msfs1; 1433Z_SHEEP; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; 14-3-3 zeta; 14-3-3 delta

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: 1: HeLa, 2: A549, 3: Neuro-2a, 4: PC-12, Protein loading quantity: 20 µg, Exposure time: 10 s, Predicted MW: 28 kDa, Observed MW: 29 kDa.

Cell line: HepG2, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% Triton X-100, Primary Ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172936.

Quick Database Links

Gene Symbol

YWHAZ