5-Propargylamino-dCTP-Cy3

SKU: orb533140

Description

5-Propargylamino-dCTP-Cy3 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incor-porated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Propargylamino-dCTP-Cy3/dCTP ratio for PCR and Nick Translation: 30-50% Propargylamino-dCTP-Cy3/70-50% dCTP.

Images & Validation

• Application Notes:
  Incorporation into DNA/cDNA by: Primer Extension with Klenow fragment; PCR with Taqpolymerase in-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: λexc 550 nm, λem 570 nm, ε 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).

Key Properties

• MW: Theoretical MW: 1132.97 g/mol (free acid); Detected MW: 1132.21 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₄₃H₅₅N₆O₂₀P₃S₂
• SMILES: N(CC#Cc1cn(c(=O)nc1N)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@H](C1)O)C(=O)CCCCCN1c2ccc(S(=O)(=O)[O-])cc2C(C)(C)/C/1=C\C=C\C1=*(c2ccc(cc2C1(C)C)S(=O)(=O)O)CC

Storage & Handling

• Storage: store at -20 °C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

5-Propargylamino-2'-deoxycytidine-5'-triphosphate, labeled with Cy3, Triethylammonium salt