8-Oxo-dGTP

SKU: orb64224

Description

8-Oxo-dGTP also known as: 8-Hydroxy-dGTP; 8-Oxo-2'-deoxyguanosine-5'-triphosphate, Sodium salt; 8-Hydroxy-2'-deoxyguanosine-5'-triphosphate, Sodium salt. 8-Oxo-dGTP is an oxidized guanine nucleotide commonly used in studies of oxidative DNA damage, nucleotide pool sanitization, and DNA repair mechanisms. As a biologically relevant oxidative lesion precursor, it is a valuable tool for investigating how damaged nucleotides affect genome stability and cellular responses under oxidative stress conditions. This modified nucleotide has been widely applied in research on base excision repair, erroneous incorporation by DNA polymerases, and hydrolysis to 8-oxo-dGMP by E. coli MutT. It is also used in studies exploring ROS-associated cellular senescence and the biological consequences of oxidized nucleotide accumulation. Owing to these features, 8-Oxo-dGTP is well suited for applications in DNA damage biology, enzymology, repair pathway analysis, and oxidative stress research.

Images & Validation

• Application Notes:
  Influence on base excision repair; Influence on erroneous incorporation by DNA-polymerases; Hydrolysis to 8-oxo-dGMP by E.-coli MutT; Triggering cell senescence through formation of ROS. ; Spectroscopic Propertie: λmax 245 nm, ε 12.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

• CAS Number: 139307-94-1
• MW: Theoretical MW: 523.18 g/mol (free acid); Detected MW: 522.99 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₁₀H₁₆N₅O₁₄P₃
• SMILES: Nc1[nH]c(=O)c2c(n(c(=O)[nH]2)[C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C2)n1

Storage & Handling

• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

8-Hydroxy-dGTP, 8-Oxo-2'-deoxyguanosine-5'-triphosphate, Sodium salt, 8-Hydroxy-2'-deoxyguanosine-5'-triphosphate, Sodium salt

Chemical structure of 8-Oxo-dGTP

Chemical structure of 8-Oxo-dGTP

Xiangxiang Wang, Yuyu Zhao, Zhaohua Hou, Xiaoxu Chen, Shuangying Jiang, Wei Liu, Xin Hu, Junbiao Dai, Guangh Large-scale pathway reconstruction and colorimetric screening accelerate cellular metabolism engineering Metabolic Engineering, 80, 107-118 (2023)

PubMed: 37717647