ABRI/ITM2B Antibody

SKU: orb1538799

Description

ABRI/ITM2B Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IF, IHC, IHC-P, WB
• Dilution Range: IF (10 µg/ml), IHC, IHC-P (2.5 - 3.75 µg/ml), Peptide-ELISA (1:8000), WB (0.1 - 0.3 µg/ml)
• Reactivity: Bovine, Equine, Human, Monkey
• Application Notes:
  Further information: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx. 33kDa band observed in Human Brain (Cerebellum and Hippocampus) lysates (calculated MW of 30.3kDa according to NP_068839.1). Recommended concentration: 1-3 ug/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-HDKETYKLQRRETIK, from the internal region of the protein sequence according to NP_068839.1.
• Target: ABRI / ITM2B
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ITM2B, ABri/ADan amyloid peptide, ABRI, BRI, BRI2, E3-16, Integral membrane protein 2B, ImBRI2, FBD, Protein E25B, BRICD2B, BRICHOS domain containing 2B, Transmembrane protein BRI, E25B, Immature BRI2

ABRI / ITM2B antibody immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

ABRI / ITM2B antibody (0.3 µg/ml) staining of HepG2 cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

ABRI / ITM2B antibody flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton.

Quick Database Links

Gene Symbol

ABRI / ITM2B