beta-Tubulin Antibody

SKU: orb44541

Description

Mouse Monoclonal to beta tubulin.

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, ICC, WB
• Reactivity: Human, Mouse, Plant, Porcine, Rat
• Application Notes:
  Immunocytochemistry: Recommended dilution: 2-5 μg/ml, fixed and permeabilized cells.Western blotting: Recommended dilution: 1-2 μg/ml, reducing conditions.Flow cytometry: Recommended dilution: 4-10 μg/ml. Intracellular staining.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgM
• Clone No.: TU-12
• Immunogen: Porcine brain microtubule protein MTP-1.
• Target: beta-Tubulin
• Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TUBB

Separation of MCF-7 cells stained using anti-beta Tubulin (TU-12) purified antibody (concentration in sample 8 µg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Quick Database Links

Gene Symbol

beta-Tubulin

UniProt

Q9H4B7

NCBI Gene ID

81027