beta-Tubulin Antibody

SKU: orb44542

Description

Mouse Monoclonal to beta tubulin.

Research Area

Cell Biology

Images & Validation

• Tested Applications: ICC, IHC-Fr, WB
• Reactivity: Human, Mouse, Plant, Porcine
• Application Notes:
  Western blotting: Recommended dilution: 1-2 μg/ml, reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgM
• Clone No.: TU-13
• Immunogen: beta-tubulin from porcine brain
• Target: beta-Tubulin
• Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TUBB

Anti-beta-Tubulin Purified (TU-13) works in WB application under reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of HeLa, HEK 293, ESS-1 and Jurkat cell lines mixed and heated (100°C, 5 min) with reducing (2-mercaptoethanol) or non-reducing SDS-loading buffer. Samples were resolved using 12% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed simultaneously with mouse IgM monoclonal antibody TU-13 (1 µg/ml) and mouse IgG1 anti-GAPDH monoclonal antibody FF26A (1 µg/ml) used as the loading control. Subclass-specific secondary antibodies IRDye 680RD Goat-anti-Mouse IgM (red) and IRDye 800CW Goat-anti-Mouse IgG (green) were used for multiplex fluorescent Western blot detection. Beta-tubulin was detected at ~50 kDa in all tested cell lines.

Quick Database Links

Gene Symbol

beta-Tubulin

UniProt

Q9H4B7

NCBI Gene ID

81027