c-FOS Antibody

SKU: orb55904

Description

Our Anti-c-FOS primary antibody from Biorbyt is mouse monoclonal. It detects bovine, human, mouse, and rat c-FOS and is Protein G purified.

Research Area

Cancer Biology, Cell Biology, Disease Biomarkers, Epigenetics & Chromatin, Neuroscience

Images & Validation

• Tested Applications: ELISA, ICC, IHC, WB
• Dilution Range: WB: 1.430555556, IHC: 0.736111111, ICC: 0.736111111
• Reactivity: Bovine, Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 2H2
• Immunogen: Recombinant full length human c-FOS expressed in and purified from E. Coli.
• Target: c-FOS
• Molecular Weight: 50
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS + 50% glycerol and 5 mM NaN3
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Activator protein 1 antibody, AP 1 antibody, C FOS antibody, Cellular oncogene c fos antibody, Cellular oncogene fos antibody, FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody, FBJ murine osteosarcoma viral oncogene homolog antibody, FBJ murine osteosarcoma viral v fos oncogene homolog antibody, FBJ Osteosarcoma Virus antibody, FOS antibody, FOS protein antibody, FOS_HUMAN antibody, G0 G1 switch regulatory protein 7 antibody, G0/G1 switch regulatory protein 7 antibody, G0S7 antibody, Oncogene FOS antibody, p55 antibody, proto oncogene c Fos antibody, Proto oncogene protein c fos antibody, Proto-oncogene c-Fos antibody, fos FBJ murine osteosarcoma viral oncogene homolog antibody

(A, B) confocal microscope images showing PV, WFA, and c-Fos (Cat. orb55904) staining after vehicle or ABC treatment in the prelimbic PFC after vehicle or ABC 31 days later. White arrows indicate triple-labelled cells. Note faint staining of PNNs after ABC treatment.

Immunofluorescence of a section of rat hippocampus showing neurons labeled with c-FOS (Cat. orb55904, 1:1000, red) counterstained with FOX3(NeuN) (green), and nuclear staining with DAPI. When both c-FOS and FOX3 are expressed the label appears orange.

Immunofluorescence of serum starved, FBS stimulated HeLa cells showing nuclear labeling of activated cells with Anti- c-FOS (Cat. orb55904, 1:1000, green) while Anti-Vimentin (Cat. orb319707, 1:500, red) labels the cytoplasmic intermediate filament, and nuclear staining with DAPI (blue).

Immunofluorescence of young male and female wild type (WT) and Alzheimer's Disease (AD) mouse brain labelling nucleus-specific c-Fos + cells in the DMH, VMH, ARH, TuN, and LH regions.

Immunolabeling of a section of rat brain that specifically labels c-FOS protein (Cat. orb55904, green, 1:1000). Frozen sections were fixed with 4%PFA for 24 hours. Before performing free floating immunohistochemistry, sections underwent antigen retrieval with sodium citrate (pH6.0) for 30 minutes at 80˚C.

Immunostaining of mouse brain labeling c-Fos protein in green (Cat. orb55904, 1:1000) and DNA with DAPI.

Representative images of CA1 region of mouse hippocampus. White arrows indicate cFos+ cells (Cat. orb55904), yellow arrows indicate 53BP1 + cells. Nuclei stained with DAPI (blue). Right: zoomed images of the white boxes.

Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa c-FOS protein.

Quick Database Links

Gene Symbol

c-FOS

UniProt

P01100