Calbindin Antibody

SKU: orb378472

Description

Our Anti-Calbindin primary antibody from Biorbyt is mouse monoclonal. It detects bovine, human, mouse, and rat Calbindin and is Protein G purified.

Research Area

Disease Biomarkers, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: WB: 1.430555556, IHC: 1:1000-1:5000
• Reactivity: Bovine, Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 5A9
• Immunogen: Recombinant full length human calbindin purified from E. coli.
• Target: Calbindin
• Molecular Weight: 28
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS + 50% glycerol and 5 mM NaN3
• Concentration: Lot Dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

avian-type antibody, CAB27 antibody, CALB 1 antibody, CALB antibody, CALB1 antibody, CALB1_HUMAN antibody, Calbindin 1 28kDa antibody, Calbindin antibody, Calbindin D28 antibody, D 28K antibody, D-28K antibody, D28K antibody, OTTHUMP00000166027 antibody, OTTHUMP00000225441 antibody, RTVL H protein antibody, Vitamin D dependent calcium binding protein antibody, Vitamin D dependent calcium binding protein avian type antibody, Vitamin D-dependent calcium-binding protein antibody

Immunofluorescence of a section of rat cerebellum labeled with anti-calbindin (Cat. orb378472, 1:2,000, red), colabeled with anti-GFAP (Cat. orb319549 , 1:5000,green), and DAPI staining of nuclear DNA. The anti-calbindin prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The anti-GFAP labels the processes of Bergmann glia in the molecular layer and the astroglia in the granular and white layers of the cerebellum.

Immunofluorescence of a section of rat cerebellum showing specific labeling of calbindin (Cat. orb378472, 1:1000, red) in the dendrites of Purkinje cells. Axons are labeled green with anti-Neurofilament H antibody (Cat. orb319585 , 1:25,000), and additional nuclear staining with DAPI.

Western blot of rat cerebellar lysate showing specific immunolabeling of the ~ 28 kDa calbindin protein.

Quick Database Links

Gene Symbol

Calbindin

UniProt

P05937