Cytokeratin 18 Mouse Monoclonal Antibody

SKU: orb1474248

Description

The Cytokeratin 18 Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Purified recombinant fragment of human Cytokeratin 18 (aa391-483) expressed in E. Coli. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB (1/500 - 1/1000), IH (1/50 - 1/100)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human Cytokeratin 18 (aa391-483) expressed in E. Coli.
• Target: KRT18
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CYK18; Keratin type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18

Western blot analysis of Cytokeratin 18 expression in A549 (A), Hela (B) whole cell lysates. (Predicted band size: 48 kD; Observed band size: 48 kD)

Immunohistochemical analysis of Cytokeratin 18 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.67). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

KRT18

UniProt

P05783

NCBI Gene ID

3875