DARPP-32 (Thr75) Antibody

SKU: orb319493

Description

Our Anti-DARPP-32 (Thr75) phosphospecific primary antibody from Biorbyt is rabbit polyclonal. It detects mouse and rat DARPP-32 (Thr75) and is antigen affinity purified.

Research Area

Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: WB: 0.736111111
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Human, Primate

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr75 of rat DARPP-32 conjugated to keyhole limpet hemocyanin (KLH).
• Target: DARPP-32 (Thr75)
• Molecular Weight: 32
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml rAlbumin and 50% glycerol.
• Concentration: Lot Dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DARPP32 antibody, DARPP-32 antibody, Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody, Dopamine and cAMP regulated neuronal phosphoprotein antibody, Dopamine and cAMP regulated phosphoprotein antibody, Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody, Dopamine and cAMP regulated phosphoprotein DARPP32 antibody, Dopamine- and cAMP-regulated neuronal phosphoprotein antibody, FLJ20940 antibody, IPPD antibody, Neuronal phosphoprotein DARPP 32 antibody, PPP1R1B antibody, PPR1B_HUMAN antibody, Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody, Protein phosphatase 1 regulatory inhibitor subunit 1B antibody, Protein phosphatase 1 regulatory subunit 1B antibody

Western blot of rat striatal lysate showing specific immunolabeling of the ~32 kDa DARPP-32 phosphorylated at Thr75 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Quick Database Links

Gene Symbol

DARPP-32 (Thr75)

UniProt

Q6J4I0