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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, ICC, WB |
|---|---|
| Dilution Range | WB: 0.736111111, ICC: 0.736111111 |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Bovine, Canine, Gallus, Human, Primate |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Sheep |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 of rat dynamin, conjugated to keyhole limpet hemocyanin (KLH). |
| Target | Dynamin (Ser774) |
| Molecular Weight | 95 |
| Purification | Antigen Affinity Purified from Pooled Serum |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml rAlbumin and 50% glycerol. |
| Concentration | Lot Dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95 kDa dynamin phosphorylated at Ser774 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
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Protocol Information
Dynamin (Ser774) Antibody (orb319507)
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