Engrailed 1 Rabbit Monoclonal Antibody

SKU: orb866424

Description

Anti-Engrailed 1 Rabbit Monoclonal Antibody. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Cell Biology, Epigenetics & Chromatin, Neuroscience, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: FC, IP, WB
• Dilution Range: WB 1:500-2000 IP 1:50 FC 1:100
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: B45G02
• Immunogen: A synthesized peptide derived from human Engrailed 1
• Target: Homeobox protein engrailed-1
• Molecular Weight: 55 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: 0.5mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ATP-binding cassette sub-family F member 1; ATP-binding cassette 50; TNF-alpha-stimulated ABC protein; ABCF1; ABC50

WB analysis of Engrailed 1 Rabbit Monoclonal Antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates. Lane 2: mouse brain tissue lysates.

Western blot analysis of Engrailed 1 expression in 293T cell lysate.

Quick Database Links

Gene Symbol

Homeobox protein engrailed-1

UniProt

Q05925