FYN (Phospho-Y530) Rabbit Polyclonal Antibody

SKU: orb213956

Description

The FYN (Phospho-Y530) Antibody is suitable for IF, IHC, IP, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y530 of human FYN protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Dilution Range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IP: 1-10-1-100
• Reactivity: Bovine, Human, Mouse, Porcine, Rat, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y530 of human FYN protein. The exact sequence is proprietary.
• Target: FYN
• Molecular Weight: 60 kDa
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tyrosine-protein kinase Fyn; Proto-oncogene Syn; Proto-oncogene c-Fyn; Src-like kinase; SLK; p59-Fyn

Western blot analysis of FYN (Phospho-Y530) expression in HEK293T (A) whole cell lysates. (Predicted band size: 60 kD; Observed band size: 60 kD)

Direct ELISA antibody dose-response curve using Anti-FYN (Phospho-Y530) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

Immunohistochemical analysis of paraffin-embedded human breast cancer using FYN (Phospho-Y530) Rabbit Polyclonal Antibody orb213956 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded human breast cancer using FYN (Phospho-Y530) Rabbit Polyclonal Antibody orb213956 with dilution at 1:100.

Quick Database Links

Gene Symbol

FYN

UniProt

P39688, P06241, Q62844

NCBI Gene ID

14360, 25150, 2534