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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 0.736111111, IHC: 0.111111111, ICC: 0.111111111 |
| Reactivity | Bovine, Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgM |
| Clone No. | 1D4 |
| Immunogen | Purified porcine GAPDH. |
| Target | Glyceraldehyde 3-Phosphate Dehydrogenase |
| Molecular Weight | 38 |
| Purification | Protein G Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS + 50% glycerol and 5 mm NaN3 |
| Concentration | 1 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Immunostaining of HeLa cells labeled with Anti-GAPDH (Cat. orb319557, 1:100, green) and nuclear staining with DAPI (blue). The anti-GAPDH produces strong cytoplasmic labeling of healthy cells.

Immunostaining of Human neuroblastoma SH-SY5Y cells labeled with Anti-GAPDH (Cat. orb319557, 1:100, green), Anti NF-H (Cat. orb319585 , red, 1:25,000) and nuclear staining with DAPI (blue).

Western blot of rat hippocampal lysate showing the specific immunolabeling of ~38 kDa GAPDH protein.
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UniProt Details
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Protocol Information
Glyceraldehyde 3-Phosphate Dehydrogenase Antibody (orb319557)
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