CD142 Antibody

SKU: orb402784

Description

Mouse monoclonal antibody to Tissue factor

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, ICC, IHC-P, WB
• Reactivity: Human
• Application Notes:
  Flow cytometry: Recommended dilution: 3-12 µg/ml.Western blotting: Recommended dilution: 1-2 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: HTF-1
• Immunogen: Human brain tissue factor (CD142)
• Target: CD142
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

F3, tissue factor, tissue thromboplastin, coagulation factor III, TF, TFA

Flow cytometry multicolor surface staining pattern of human PHA stimulated peripheral blood mononuclear cells using anti-human CD142 (HTF-1) purified antibody (concentration in sample 4 μg/ml, GAM APC) and SYTO 16.

Separation of human CD142 positive lymphocytes (red-filled) from CD142 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD142 (HTF-1) purified antibody (concentration in sample 4 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD142 (HTF-1) purified antibody (concentration in sample 4 μg/ml, GAM APC).

Immunohistochemistry staining of human cortex (paraffin-embedded sections) with anti-CD142 (HTF-1), 10 μg/ml.

Western blotting analysis of human CD142 using mouse monoclonal antibody HTF-1 on lysates of A431 cell line and MCF7 and THP1 cell lines (negative controls) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD142 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD142 was detected around 50 kDa.

Quick Database Links

Gene Symbol

CD142

UniProt

P13726

NCBI Gene ID

2152