Lactoperoxidase Antibody Biotin Conjugated

SKU: orb345052

Description

Lactoperoxidase antibody (Biotin)

Images & Validation

• Tested Applications: DOT, ELISA, IP, WB
• Dilution Range: ELISA: 1:500 - 1:2,000, IP: 1:100, WB: 1:50 - 1:500
• Reactivity: Bovine
• Application Notes:
  Anti-Lactoperoxidase Biotin Conjugated Antibody has been tested by Dot blot and western blot and is suitable to be assayed against 1.0 ug of Lactoperoxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:6,000 of the reconstitution concentration is suggested for Anti-Lactoperoxidase. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 100 µL

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Key Properties

• Antibody Type: Primary Antibody
• Host: Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Lactoperoxidase [Bovine Milk]
• Target: LPO
• Purity: Anti-Lactoperoxidase (Bovine Milk) (Sheep) Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Lactoperoxidase [Bovine Milk]. Cross reactivity against Lactoperoxidase from other sources is unknown.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

sheep anti-Lactoperoxidase biotin conjugated Antibody, biotin conjugated sheep anti-Lactoperoxidase Antibody, LPO antibody, Salivary peroxidase antibody, SAPX antibody, SPO antibody

Biorbyt Sheep anti Lactoperoxidase antibody was used to detect Lactoperoxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in orb348637). Detection shown was using Dylight 488 conjugated secondary antibody (1:10K in TBS/orb348637 1 hr RT).

Quick Database Links

Gene Symbol

LPO

UniProt

P80025

RefSeq (Protein):NP_776358.1