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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 3.513888889, IHC: 1.430555556, ICC: 1:500-1:2000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 5H6 |
| Immunogen | Recombinant full length human LAMP1 expressed in and purified from E. coli. |
| Target | LAMP1 |
| Molecular Weight | 100 |
| Purification | Protein G Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS + 10 mM NaN3. |
| Concentration | 1 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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mmunostaining analysis of HeLa cells lysate using LAMP1 antibody

Immunolabeling analysis of HeLa cells lysate using LAMP1 antibody

Western blot analysis of HeLa cell lysate using LAMP1 antibody

Immunolabeling of HeLa cells showing vesicular staining of LAMP1 protein (Cat. orb319565, red, 1:2000) accumulated in swollen lysosomes, while Anti-Vimentin (Cat. orb319707, green, 1:500) specifically labels the intermediate filament network in these cells. The blue stain is DAPI to reveal nuclear DNA.

Immunostaining of HeLa cells showing specific, strong cytoplasmic labeling of LAMP1 (Cat. orb319565, red, 1:2000) corresponding to lysosomes and late endosomes. Additional staining with Anti-Vimentin (Cat. orb319707, green, 1:500) specifically labeling the intermediate filament network. The blue stain is DAPI to reveal nuclear DNA.

Western blot of HeLa cell lysate showing specific immunolabeling of the ~100 kDa LAMP1 protein.
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LAMP1 Antibody (orb319565)
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