Microtubule Associated Protein 2 (MAP2) Antibody

SKU: orb613679

Description

Our Anti-Microtubule Associated Protein 2 (MAP2) primary antibody from Biorbyt is goat polyclonal. It detects human, mouse, and rat Microtubule Associated Protein 2 (MAP2) and is Protein G purified.

Research Area

Disease Biomarkers, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA, ICC, IHC, WB
• Dilution Range: WB: 0.736111111, IHC: 1:1000-1:2000, ICC: 1:1000-1:2000
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant human MAP2 protein expressed in and purified from E. Coli.
• Target: Microtubule Associated Protein 2 (MAP2)
• Molecular Weight: 280
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS + 50% glycerol and 5 mM NaN3
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DKFZp686I2148 antibody, MAP 2 antibody, MAP dendrite specific antibody, MAP-2 antibody, MAP2 antibody, MAP2A antibody, MAP2B antibody, MAP2C antibody, Microtubule associated protein 2 antibody, Microtubule-associated protein 2 antibody, MTAP2_HUMAN antibody

Immunofluorescence of a section of rat brain stem showing specific labeling of MAP2 (Cat. orb613679, 1:2000, red) in the perikarya and dendrites of neurons and specific labeling of the myelin sheath around axons with anti-MBP (Cat. orb319582 , 1:5000, green). The blue is DAPI revealing nuclear DNA.

Immunofluorescent image of primary mouse neurons specifically labelling MAP2 (Cat. orb613679, 1:1000, red). The blue nuclear stain is Hoechst.

Immunolabeling for GSK3β (green) and MAP2 (Cat. orb613679; magenta) in hippocampal CA1 neurons in 9-month-old 3xTg and 3xTg; cKO mice.

Immunolabeling of REST (green) and neuron marker MAP2 (Cat. orb613679; red) in 3xTg primary cortical neurons (PCNs) shows nuclear REST in neurons treated with vehicle and decreased nuclear REST in 3xTg neurons after a 24-h treatment with the Wnt/β-catenin inhibitors Dickkopf 1 (DKK1), XAV939 and ICG001, or the PERK inhibitor GSK2606414.

Immunolabeling of REST (green), the neuron marker MAP2 (Cat. orb613679; magenta) and nuclei (DAPI, blue) in the CA1 region of the hippocampus, shows elevated nuclear REST levels in 3-month-old J20 mice.

Western blot of rat cortical lysate showing specific immunolabeling of the ~280 kDa MAP2 protein.

Quick Database Links

Gene Symbol

Microtubule Associated Protein 2 (MAP2)

UniProt

P11137