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Description
Research Area
Images & Validation
−| Tested Applications | FC, ICC, IHC-Fr |
|---|---|
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Rat IgG2a kappa |
| Clone No. | 5H10-27 (MFR5) |
| Immunogen | C57BL/6 x A/J)F1 murine mast cell line |
| Target | CD49e |
| Purification | Purified by protein-G affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Separation of murine bone marrow cells stained using anti-mouse CD49e (5H10-27(MFR5)) purified antibody (concentration in sample 0.56 μg/ml, DAR APC) from murine bone marrow cells unstained by primary antibody (DAR APC, black-dashed) in flow cytometry analysis (surface staining).

Flow cytometry surface staining pattern of murine bone marrow using anti-mouse CD49e (5H10-27(MFR5)) purified antibody (concentration in sample 0.56 μg/ml, DAR APC).
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Protocol Information
CD49e Antibody (orb623566)
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![Anti-alpha 5 beta 1 Integrin [M200 (Volociximab)]](/images/pub/media/catalog/product/NewWebsite/35/orb348884_1.png)