Pyruvate Kinase Antibody Biotin Conjugated

SKU: orb344384

Description

Pyruvate Kinase antibody (Biotin)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:1,000 - 1:5,000, WB: 1:200 - 1:1,000
• Reactivity: Rabbit
• Application Notes:
  Anti-Pyruvate Kinase Biotin Antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Pyruvate Kinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,000 of the reconstitution concentration is suggested for Anti-Pyruvate Kinase Antibody. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 100 µL

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Pyruvate Kinase [Rabbit Muscle]
• Target: PKM
• Purity: Anti-Pyruvate Kinase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle]. Anti-Pyruvate Kinase has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross reactivity against Pyruvate Kinase from other sources may occur but have not been specifically determined.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat anti-Pyruvate Kinase Antibody, biotin Conjugated goat anti-Pyruvate Kinase Antibody, Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase 2/3 antibody, Cytosolic thyroid hormone-binding protein antibody, PKM2

Western Blot of Goat Anti-Pyruvate Kinase Biotin Conjugate. Lane 1: Opal Prestain Molecular Weight Marker. Lane 2: 50 ng of Pyruvate Kinase. Primary Antibody: Goat Anti-Pyruvate Kinase Biotin at 1 ug/ml o/n @ 4C. Secondary Antibody: Streptavidin-HRP: orb348767 at 1:40000 for 30 min at RT. Blocking: orb348637 for 30 min at RT.

Quick Database Links

Gene Symbol

PKM

UniProt

P11974

RefSeq (Protein):NP_001182574.1