Pyruvate Kinase Antibody Biotin Conjugated

SKU: orb344385

Description

Pyruvate Kinase antibody (Biotin)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: WB: 1:200 - 1:1,000
• Reactivity: Rabbit
• Application Notes:
  Anti-Pyruvate Kinase Biotin Antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Pyruvate Kinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,000 of the reconstitution concentration is suggested for Anti-Pyruvate Kinase Antibody

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Pyruvate Kinase [Rabbit Muscle]
• Target: PKM
• Purity: Anti-Pyruvate Kinase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle]. Anti-Pyruvate Kinase has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross reactivity against Pyruvate Kinase from other sources may occur but have not been specifically determined.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

goat anti-Pyruvate Kinase Antibody, biotin Conjugated goat anti-Pyruvate Kinase Antibody, Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase 2/3 antibody, Cytosolic thyroid hormone-binding protein antibody, PKM2

Western Blot of Goat Anti-Pyruvate Kinase Biotin Conjugate. Lane 1: Opal Prestain Molecular Weight Marker. Lane 2: 50 ng of Pyruvate Kinase. Primary Antibody: Goat Anti-Pyruvate Kinase Biotin at 1 ug/ml o/n @ 4C. Secondary Antibody: Streptavidin-HRP: orb348767 at 1:40000 for 30 min at RT. Blocking: orb348637 for 30 min at RT.

Quick Database Links

Gene Symbol

PKM

UniProt

P11974

RefSeq (Protein):NP_001182574.1