RAB27 Rabbit Polyclonal Antibody

SKU: orb381996

Description

The RAB27 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human RAB27 .Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:500-2000, IHC-P: 1:50-200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human RAB27
• Target: RAB27A
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RAB27; Ras-related protein Rab-27A; Rab-27; GTP-binding protein Ram

Western blot analysis of RAB27 expression in Hela (A), U937 (B), Jurkat (C) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 25 kD)

Immunofluorescent analysis of RAB27 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

RAB27A

UniProt

P51159, P23640, Q9ERI2

NCBI Gene ID

5873, 11891, 50645

Simon Heidegger,Florian Stritzke,Sarah Dahl,Juliane Dabler-Plenker,Laura Joachim,Dominik Buschmann,Kaiji Fan,Carolin M. Sauer,Nils Ludwig,Christof Winter,Stefan Enssle,Suqi Li,Markus Perl,Andre Gorgens,Tobias Haas,Erik Thiele Orberg,Sascha Gottert,Catherine Wolfel,Thomas Engleitner,Isidro Cortes-Ciriano,Roland Rad,Wolfgang Herr,Bernd Giebel,Jurgen Ruland,Florian Bassermann,Christoph Coch,Gunther Hartmann,and Hendrik Poeck Targeting nucleic acid sensors in tumor cells to reprogram biogenesis and RNA cargo of extracellular vesicles for T cell-mediated cancer immunotherapy Cell Reports Medicine, 4, 101171 (2023)