TCR alpha/beta Antibody (FITC)

SKU: orb154478

Description

Mouse monoclonal antibody conjugated to FITC which recognizes TCR alpha/beta that recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either alpha and beta or gamma. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex.

Images & Validation

• Tested Applications: FC
• Reactivity: Primate, Rat
• Application Notes:
  Flow cytometry: Recommended dilution: 1-3 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: R73
• Immunogen: Rat T blasts and erythrocytes
• Target: TCR alpha/beta
• Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TCRA/B

Flow cytometry analysis (surface staining) of rat splenocytes with anti-rat TCR alpha/beta (R73) FITC.

Quick Database Links

Gene Symbol

TCR alpha/beta

NCBI Gene ID

35