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Description
Research Area
Cancer Biology
Images & Validation
−| Tested Applications | WB |
|---|---|
| Dilution Range | WB (1/500 - 1/1000) |
| Reactivity | Hamster, Human, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S423/425 of human SMAD3 protein. The exact sequence is proprietary. |
| Target | SMAD3 |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

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Western blot analysis of SMAD3 (Phospho-S423/425) expression in Jurkat (A), C6 (B), CHOK1 (C), Hela (D) whole cell lysates. (Predicted band size: 48 kD; Observed band size: 52 kD)
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
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Datasheet
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SMAD3 (Phospho-S423/425) Rabbit Monoclonal Antibody (orb1474582)
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