SYK Antibody

SKU: orb44501

Description

Mouse Monoclonal to Syk.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ICC, IHC-P, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Western blotting: Recommended dilution: 1-2 μg/ml.Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml; positive tissue: tonsil B cells.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: SYK-01
• Immunogen: Recombinant fragment (aa 5-360) of human Syk.
• Target: SYK
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

p72Syk

Immunocytochemistry analysis of Syk in human primary fibroblasts using anti-Syk (SYK-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Immunocytochemistry analysis of Syk in human HeLa cell line using anti-Syk (SYK-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Western blotting analysis of human Syk using mouse monoclonal antibody SYK-01 on lysates of Ramos and Jurkat (negative control) cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-Syk monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. Syk was detected around 72 kDa.

Quick Database Links

Gene Symbol

SYK

UniProt

P43405

NCBI Gene ID

6850