Synaptotagmin (Thr202) Antibody

SKU: orb319676

Description

Our Anti-Synaptotagmin (Thr202) rabbit polyclonal phosphospecific primary antibody from Biorbyt is produced in-house. It detects rat Synaptotagmin (Thr202) and is antigen affinity purified from pooled serum.

Research Area

Cell Biology, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA, ICC, WB
• Dilution Range: WB: 0.736111111, ICC: 0.319444444
• Reactivity: Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Human, Mouse, Primate, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202 of rat synaptotagmin, conjugated to keyhole limpet hemocyanin (KLH).
• Target: Synaptotagmin (Thr202)
• Molecular Weight: 62
• Purification: Antigen Affinity Purified from Pooled Serum
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml rAlbumin and 50% glycerol.
• Concentration: Lot Dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DKFZp781D2042 antibody, FLJ42519 antibody, P65 antibody, SVP65 antibody, synaptotagmin 1 antibody, Synaptotagmin I antibody, SYT antibody, SYT1 antibody, SytI antibody

Immunostaining of 14 DIV rat cortical neurons labeling synaptotagmin when phosphorylated at Thr202 (Cat. orb319676, 1:400, red) and PSD95 (green). The cortical neurons were fixed with methanol at -20C for 10 minutes.

Western blot of rat cortical lysate showing specific immunolabeling of the ~62 kDa synaptotagmin phosphorylated at Thr202 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Quick Database Links

Gene Symbol

Synaptotagmin (Thr202)

UniProt

P21707