APOB Antibody

SKU: orb1260491

Description

APOB Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:200 - 1:2000IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 28-330 of human APOB (NP_000375.2).
• Target: APOB
• Molecular Weight: Observed: 110kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

APOB, apolipoprotein B (including Ag(x) antigen), FLDB, apoB-100, apoB-48, apolipoprotein B, apolipoprotein B48, LDLCQ4

Western blot analysis of extracts of various cell lines, using APOB antibody (orb1260491) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunofluorescence analysis of C6 cells using APOB antibody (orb1260491) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using APOB antibody (orb1260491) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

APOB

UniProt

P04114