APOB Antibody

SKU: orb345277

Description

APOLIPOPROTEIN B antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IHC, IP, WB
• Dilution Range: ELISA: 1:2,000 - 1:10,000, IHC: 1:50 - 1:500, IP: 1:100, WB: 1:200 - 1:1,000
• Reactivity: Human
• Application Notes:
  Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: apoLipoprotein Type B was isolated from human plasma by density gradient centrifugation followed by HPLC purification.
• Target: APOB
• Purity: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat anti-Apolipoprotein B antibody, Apo B-100, Apo B-48, APOB protein antibody, Apolipoprotein-B 100 antibody, Apolipoprotein B 48 antibody, Apolipoprotein B antibody, FLDB antibody

LDs are present in the lumen of the type I NR. a Huh7 treated with OA/TM for 24–48 h harbored LDs in the lumen of the ER (left; arrowheads) and within the type I NR (right; arrows mark the NR). INM: inner nuclear membrane, ONM: outer nuclear membrane. Bars, 0.2 µm. b Huh7-expressing HRP-KDEL treated with OA/TM for 24 h. DAB precipitated in the type I NR lumen (arrows). Bar, 0.5 µm. c Mouse hepatocytes in vivo after high-fat diet feeding for 6 weeks and TM injection. Lumenal LDs were observed in the ER (arrowheads), the nuclear cistern (arrowhead in the inset) (left figure; Bar, 0.5 µm), and in the type I NR (arrows mark the NR) (right figure; Bar, 0.2 µm). They contained more nuclear LDs than the control fed the high-fat diet and injected with vehicle alone. Mean ± SD of three independent experiments. *p < 0.01, Student's t test. d Microsomes of Huh7 treated with none, 0.4 mm OA, 1.2 mm OA, or OA/TM for 48 h. The OA/TM-treated cell microsome contained triglycerides (TG) and cholesterol esters (CE) most abundantly (by thin layer chromatography), but showed the lowest amount of ApoB (by Western blotting). e Three different kinds of LDs in the nuclear area: Nucleoplasmic LDs (A), NR-lumenal LDs (within the type I NR) (B), and cytoplasmic LDs (within the type II NR) (C). f Nucleoplasmic LDs (arrowheads) and NR-lumenal LDs (arrows) are distinguished by whether they are outside of or within LBR rings, respectively. Huh7 treated with OA/TM for 48 h. Both LDs were reduced by MTPi (100 nm BAY 13-9952). Bar, 10 µm. See also Supplementary Fig. 2e. g The number of nucleoplasmic LDs, NR-lumenal LDs, and cytoplasmic LDs within the type II NR were counted in randomly taken electron micrographs of Huh7 treated with OA/TM for 48–72 h. Box plot of pooled data from three independent experiments. The average is shown by +. Number of nuclei examined = 128.

Quick Database Links

Gene Symbol

APOB

UniProt

Q7Z7Q0

RefSeq:AAH51278.1