ASAH1 Antibody

SKU: orb1239216

Description

ASAH1 Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine
• Application Notes:
  ASAH1 antibody can be used for detection of ASAH1 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human and rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: ASAH1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of the human ASAH1.The immunogen is located within amino acids 240 - 290 of ASAH1.
• Target: ASAH1
• Molecular Weight: Predicted: 45 kDaObserved: 49 kDa
• Purification: ASAH1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: ASAH1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ASAH1 Antibody: AC, PHP, ASAH, PHP32, ACDase, SMAPME, HSD-33, HSD33, Acid ceramidase, Acylsphingosine deacylase, AC

Western blot analysis of ASAH1 in human heart tissue lysate with ASAH1 antibody at 1 µg/mL.

Immunohistochemistry of ASAH1 in human heart tissue with ASAH1 antibody at 2.5 µg/mL.

Immunofluorescence of ASAH1 in Human Heart cells with ASAH1 antibody at 20 µg/mL.

Immunohistochemistry of ASAH1 in rat heart tissue with ASAH1 antibody at 5 µg/mL.

Immunofluorescence of ASAH1 in rat heart tissue with ASAH1 antibody at 20 µg/mL. Red: ASAH1 Antibody (orb1239216) Blue: DAPI staining.

Quick Database Links

Gene Symbol

ASAH1

UniProt

Q13510

RefSeq:EAW63795