ASB10 Rabbit Polyclonal Antibody

SKU: orb100724

Description

ASB10 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ASB10 (401-450/467aa)
• Target: ASB10
• Molecular Weight: 51 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GLC1F; ASB10_HUMAN; ASB10; ASB-10; ankyrin repeat and SOCS box containing 10; ankyrin repeat and SOCS box-containing 10; glaucoma 1, open angle, F (adult-onset)

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ASB10) Polyclonal Antibody, Unconjugated (orb100724) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-ASB10 (orb100724) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kD, Observed band size: 53 kD.

Sample: Lane 1: Mouse Heart tissue lysates, Lane 2: Mouse Muscle tissue lysates, Primary: Anti-ASB10 (orb100724) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kDa, Observed band size: 51 kDa.

Quick Database Links

Gene Symbol

ASB10

UniProt

Q8WXI3