Avi Epitope Tag Antibody

SKU: orb750518

Description

BirA antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:5,000, WB: 1:5,000
• Application Notes:
  Anti-Biotin Ligase Epitope Tag Antibody has been tested in ELISA and Western Blot. Western blot analysis of B. subtilis strains producing either (BAP-tagged) SPoIIIAH (XWB49) or (BAP-tagged) SpoIIQ (XWB46) showed strong and specific reactivity to the antisera. Minor background bands of unknown identity may be observed. The antisera will recognize the BAP/BLT tag irrespective of the whether the tag is biotinylated (data not shown).

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: Anti-Avi Epitope Tag antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic avi-tag peptide GLNDIFEAQKIEWH (minimal BirA substrate; biotin is added to the sequence at the lysine).
• Purity: This antiserum detects the minimal peptide substrate GLNDIFEAQKIEWH for the E. coli biotin ligase BirA. Western blot analysis of B. subtilis strains producing either (Avi-tagged) SPoIIIAH (XWB49) or (Avi-tagged) SpoIIQ (XWB46) showed strong and specific reactivity to the antisera.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 90 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Biotin Ligase Epitope Tag Antibody, rabbit anti-Avi-tag antibody, biotin ligase, biotin operon repressor protein, biotin holoenzyme synthetase, biotin-[acetyl-CoA carboxylase] synthetase

Western blot using Biorbyt's Anti-Biotin Ligase Epitope Tag antibody shows detection of the BLT Ligase Target in lysates of whole cell Bacillus subtilis strains producing either BAP-tagged (Biotin-Acceptor Peptide-tagged) SpoIIIAH (XWB49) (~ 25 kDa) or BAP-tagged SpoIIQ (XWB46) (~33 kDa). These proteins regulate transcription in Bacillus subtilis. The identity of the lower molecular weight bands in the last lane is unknown.