Bcl-6 Rabbit Polyclonal Antibody

SKU: orb4674

Description

Bcl-6 Rabbit Polyclonal Antibody

Research Area

Cancer Research, DNA and RNA Research, Proto-oncogenes, Transcription Factors, Zinc Finger Protein

Images & Validation

• Tested Applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Equine, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Bcl-6 (331-430/706aa)
• Target: BCL6
• Molecular Weight: 98 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BCL5; BCL6A; LAZ3; ZBTB27; ZNF51; BCL6_HUMAN; BCL6; BCL-6; B-cell lymphoma 5 protein (BCL-5); Protein LAZ-3; Zinc finger and BTB domain-containing protein 27; Zinc finger protein 51; BCL6_MOUSE; BCL6 transcription repressor; B cell CLL/lymphoma 6; BCL6, transcription repressor

Sample: Lane 1: Human RAMOS cell lysates, Lane 2: Human Raji cell lysates, Lane 3: Human Jurkat cell lysates, Primary: Anti-Bcl-6 (orb4674) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kDa, Observed band size: 100 kDa.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Bcl-6) Polyclonal Antibody, Unconjugated (orb4674) at 1:25 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Bcl-6) Polyclonal Antibody, Unconjugated (orb4674) at 1:25 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

BCL6

UniProt

P41182