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Description
Research Area
Images & Validation
−| Tested Applications | FC, IHC-P, WB |
|---|---|
| Reactivity | Human |
| Predicted Reactivity | Bovine, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Immunogen | This BGN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-176 amino acids from the Central region of human BGN. |
| Target | BGN |
| Molecular Weight | 42 kDa |
| Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Biglycan Rabbit Polyclonal Antibody [orb100396]
IF, IHC-Fr, IHC-P, WB
Bovine, Canine, Equine, Porcine, Rabbit, Rat, Sheep
Human, Mouse
Rabbit
Polyclonal
Unconjugated
Rat Biglycan (BGN) ELISA Kit [orb781567]
Rat
1.57-100 ng/mL
0.65 ng/mL
Human Biglycan (BGN) ELISA Kit [orb777300]
Human
0.32-20 ng/mL
0.131 ng/mL
Mouse Biglycan (BGN) ELISA Kit [orb780339]
Mouse
3.13-200 ng/mL
1.41 ng/mL
Biglycan Preproprotein Antibody [orb19394]
ELISA, IF
Canine, Rat
Human, Mouse
Polyclonal
Unconjugated

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Western blot analysis of BGN using rabbit polyclonal BGN Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BGN gene.

Western blot analysis of BGN Antibody in Sk-Br-3 cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human brain tissue reacted with BGN Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Protocol Information
BGN Antibody (orb1263682)
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