Biotin-16-dCTP

SKU: orb64049

Description

Biotin-16-dCTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detec-tion of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of cytidine. Recommended Biotin-16-dCTP/dCTP ratio for PCR: 50% Biotin-16-dCTP/50% dCTP.

Images & Validation

• Application Notes:
  Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase I; Primer Extension with Klenow exo-; 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) ; Reverse Transcription with MMLV Reverse Transcriptase. Spectroscopic Propertie: λmax 294 nm, ε 9.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

  Read more...

Key Properties

• MW: Theoretical MW: 944.78 g/mol (free acid); Detected MW: 944.23 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₃₂H₅₁N₈O₁₇P₃S (free acid)

Storage & Handling

• Storage: store at -20 °C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Biotin-16-Propargylamino-dCTP, γ-[N-(Biotin-6-amino-hexanoyl-6-aminobutanoyl)]-5-(3-propargylamino)-2'-deoxycytidine-5'-triphosphate, Triethylammonium salt

Chemical structure of using Biotin-16-dCTP (Biotin) (Small Molecules)