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Description
Research Area
Images & Validation
−| Tested Applications | IHC, IHC-P, WB |
|---|---|
| Dilution Range | IHC, IHC-P (10 µg/ml), WB (1:2000) |
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone No. | 4C2 |
| Immunogen | Full length human recombinant protein of human BTLA(NP_861445) produced in HEK293T cell. |
| Target | BTLA / CD272 |
| Purification | Protein A/G purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% rAlbumin |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Anti-BTLA / CD272 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTLA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTLA.
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BTLA/CD272 Antibody (orb1539457)
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![Anti-BTLA [HMBT-6B2]](/images/pub/media/catalog/product/NewWebsite/35/orb613960_1.png)
![Anti-BTLA [HMBT-6B2]](/images/pub/media/catalog/product/NewWebsite/35/orb613961_1.png)
