CAT Antibody

SKU: orb344573

Description

Catalase antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000, WB: 1:10,000 - 1:50,000
• Reactivity: Bovine, Human, Mouse, Rat, Sheep
• Application Notes:
  Anti-Catalase is tested for use in ELISA, western blot, and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Catalase [Bovine Liver]
• Target: CAT
• Purity: Anti-Catalase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Catalase [Bovine liver]. Cross reactivity against Catalase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Catalase Antibody, Cas 1 antibody, CAT antibody, Cs1 antibody, MGC138422 antibody, MGC138424 antibody

Immunohistochemistry of Catalase antibody. Tissue: human liver. Fixation: formalin fixed paraffin embedded. Antigen retrieval: user optimized. Primary antibody: Catalase antibody 1:400. Secondary antibody: Peroxidase goat anti-rabbit at 1:10000 for 45 min at RT. Localization: nuclear and occasionally cytoplasmic. Staining: Liver parenchyma: portal triads affected by moderate lymphoplasmacytic inflammation with minimal proliferation of bile ductules. Not obvious interstitial fibrosis. Cytoplasma of hepatocytes, diffuse positivity for the antibody anti-catalase. Neg Ctr (far right) normal rabbit IgG with Human liver. pH6.2 40X

Western Blot of Rabbit anti-Catalase antibody. Lane R: Reduced samples of purified Catalase. Lane NR: Non-reduced samples of purified Catalase. Load: ~1 ug of protein per lane. Primary antibody: Catalase antibody at 1:1000 for overnight at 4°C. Secondary antibody: Dylight488™ rabbit secondary antibody at 1:10000 for 1.5 hrs at RT. Block: orb348637 overnight at 4°C. Predicted/Observed size: 59.9 kDa, 55 kDa for catalase. Other band(s): none.

Quick Database Links

Gene Symbol

CAT

UniProt

P00432

RefSeq:AAI03067.1