CAT Antibody

SKU: orb750575

Description

CAT antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:100,000 - 1:1,000,000, WB: 1:5,000 - 1:20,000
• Reactivity: Bovine, Human, Mouse, Rat, Sheep
• Application Notes:
  Anti-Catalase Antibody has been tested by western blotting and is suitable for IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: Anti-Catalase Antibody was produced by repeated immunizations with bovine liver catalase protein.
• Target: CAT
• Purity: Anti-Catalase Antibody was prepared from monospecific antiserum by delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Catalase [Bovine Liver]. Cross reactivity against Catalase from other tissues and species may occur but has not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store Catalase Antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 85 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Catalase Antibody, Cas 1 antibody, CAT antibody, Cs1 antibody, MGC138422 antibody, MGC138424 antibody

Western Blot of Rabbit Anti-Catalase (Bovine Liver) Antibody. Lane 1: CHO K1 Lysate 10 µg. Lane 2: CHO S Lysate 10 µg. Lane 3: Opal Pre-stained Molecular Weight Marker. Primary Antibody: Rabbit Anti-Catalase at 1:500 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG CY5 at 1:10000 for 30 in at RT. Expect: ~59.8kDa.

Western Blot of Rabbit anti-Catalase antibody. Lane R: Reduced samples of purified Catalase. Lane NR: Non-reduced samples of purified Catalase. Load: ~1 ug of protein per lane. Primary antibody: Catalase antibody at 1:1000 for overnight at 4°C. Secondary antibody: Dylight488™ rabbit secondary antibody at 1:10000 for 1.5 hrs at RT. Block: orb348637 overnight at 4°C. Predicted/Observed size: 59.9 kDa, 55 kDa for catalase. Other band(s): none.

Quick Database Links

Gene Symbol

CAT

UniProt

P00432

RefSeq:AAI03067.1