CD137L Antibody / 4-1BBL / TNFSF9

SKU: orb640013

Description

TNFSF9 / 4-1BBL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9 / 4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9 / 4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. It has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine is required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.

Images & Validation

• Tested Applications: FACS, IHC-P
• Dilution Range: Flow cytometry: 1-2ug/10^6 cells in 0.1ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the CD137L antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: CD137L/1547
• Immunogen: A recombinant full-length human CD137L protein was used as the immunogen for the CD137L antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human renal cell carcinoma with CD137L antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human renal cell carcinoma with CD137L antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Flow cytometry testing of PFA-fixed human 293 cells with CD137L antibody; Red=isotype control, Blue= CD137L antibody.

SDS-PAGE analysis of purified, BSA-free CD137L antibody as confirmation of integrity and purity.

Quick Database Links

UniProt

P41273