CD275/B7-H2/ICOS Ligand Antibody

SKU: orb1537722

Description

CD275/B7-H2/ICOS Ligand Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, IHC, IHC-P
• Dilution Range: Flo (0.5 µg/10E6 cells), IHC, IHC-P (1:50)
• Reactivity: Human
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 1:50.

  Read more...

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone No.: 2D3
• Immunogen: Full-length human B7H2-mIg fusion protein.
• Target: CD275 / B7-H2 / ICOS Ligand
• Purification: Affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.2, 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ICOSLG, B7RP1, B7 homolog 2, B7 homologue 2, B7-H2, B7H2, B7RP-1, CD275 antigen, ICOS ligand, GL50, ICOS-L, ICOSL, KIAA0653, B7-like protein Gl50, B7-related protein 1, CD275, LICOS

Human monocyte-derived dendritic cells stained with purified 2D3, then detected with biotinylated anti-mouse IgGs and Sav-PE. Antibody used ad 0.5 ug/million cells in 100 ul volume.

Quick Database Links

Gene Symbol

CD275 / B7-H2 / ICOS Ligand