CD34 Antibody

SKU: orb1248643

Description

CD34 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 85+95kDa bands observed in Human Peripheral Blood Mononucleocytes lysates (calculated MW of 35.0kDa according to NP_001764.1). The observed MW correspond to glycosylated forms. Recommended concentration: 1-3µg/mL. Primary incubation was 1 hour.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-SPTGERLELEP
• Target: CD34
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD34, CD34 molecule, CD34 antigen, OTTHUMP00000034733, OTTHUMP00000034734, hematopoietic progenitor cell antigen CD34, CD34-T

orb1248643 (2 ug/ml) staining of Peripheral Blood Lymphocytes lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Flow Cytometry analysis of paraformaldehyde fixed Jurkat using orb1248643 at 10 ug/ml in green and negative control goat IgG at 0.1 mg/ml in blue. Detected by Alexa Fluor?488. This data is from a previous batch, not on sale.

Quick Database Links

Gene Symbol

CD34

UniProt

P28906

RefSeq (Protein):NP_001764.1