HCAM Antibody / CD44

SKU: orb749333

Description

This antibody recognizes a cell surface glycoprotein of 80-95kDa, called CD44, or HCAM, on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44-s). Higher molecular weight isoforms are described in epithelial cells (CD44-v), which are believed to function in intercellular adhesion and stromal binding. HCAM antibody immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Flow cytometry: 2-4ug/10^6 cells,Immunofluorescence: 2-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HCAM antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: 156-3C11
• Immunogen: Stimulated human leukocytes were used as the immunogen for this CD44 / HCAM antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Immunofluorescent staining of human HeLa cells with HCAM antibody (clone 156-3C11, green) and Reddot nuclear stain (red).

IHC testing of FFPE human tonsil stained with HCAM antibody (clone 156-3C11).

IHC testing of FFPE human breast cancer stained with HCAM antibody (clone 156-3C11).

Western blot testing of HeLa cell lysate with HCAM antibody (clone 156-3C11). Predicted molecular weight ~81 kDa.

SDS-PAGE analysis of purified, BSA-free HCAM antibody (clone 156-3C11) as confirmation of integrity and purity.