CD62p Recombinant Rabbit Monoclonal Antibody

SKU: orb1152036

Description

CD62p Recombinant Rabbit Monoclonal Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG/Kappa
• Clone No.: B42E38
• Immunogen: A synthesized peptide derived from human CD62P (800-830aa)
• Target: SELP
• Molecular Weight: 140/90 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD62; CD62P; GMP140; GRMP; LECAM3; PADGEM; PSEL; GMP-140; LYAM3_HUMAN; SELP; CD62 antigen-like family member P; Granule membrane protein 140 (GMP-140); Leukocyte-endothelial cell adhesion molecule 3 (LECAM3); Platelet activation dependent granule-external membrane protein (PADGEM); GMRP; LYAM3_MOUSE; selectin P; selectin P (granule membrane protein 140kD, antigen CD62); selectin P (granule membrane protein 140kDa, antigen CD62); P-selectin; antigen CD62; granule membrane protein 140kDa; platelet activation-dependent granule to external membrane protein

25 ug total protein per lane of various lysates probed with CD62p monoclonal antibody, unconjugated (orb1152036) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded Human Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD62p Monoclonal Antibody, Unconjugated (orb1152036) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Esophagus, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD62p Monoclonal Antibody, Unconjugated (orb1152036) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Lung, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD62p Monoclonal Antibody, Unconjugated (orb1152036) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD62p Monoclonal Antibody, Unconjugated (orb1152036) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Tonsil, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD62p Monoclonal Antibody, Unconjugated (orb1152036) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD62p Monoclonal Antibody, Unconjugated (orb1152036) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Quick Database Links

Gene Symbol

SELP

UniProt

P16109