CD73 Antibody

SKU: orb607134

Description

The protein encoded by the NT5E gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene. [RefSeq]

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FACS, IF, IHC-P, WB
• Dilution Range: ELISA: order BSA-free format for coating,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Reactivity: Human
• Application Notes:
  Optimal dilution of the CD73 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: CDLA73-1
• Immunogen: A recombinant full length human NT5E protein was used as the immunogen for the CD73 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Flow cytometry testing of human U-87 MG cells with CD73 antibody (clone CDLA73-1); Red=isotype control, Blue= CD73 antibody.

Immunofluorescence staining of human U-87 MG cells with CD73 antibody (green, clone CDLA73-1) and Reddot nuclear stain (red).

IHC staining of FFPE human tonsil tissue with CD73 antibody (clone CDLA73-1). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of human 1) A431 and 2) PC-3 cell lysate with CD73 antibody (clone CDLA73-1). Predicted molecular weight: 60-70 kDa.

Quick Database Links

UniProt

P21589