CDKN1B Rabbit Polyclonal Antibody

SKU: orb395397

Description

Rabbit polyclonal antibody to KIP1.

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC, IP, WB
• Dilution Range: WB: 1:200-1:2000; IP: 1:200-1:1000; IHC: 1:50-1:500; IF: 1:50-1:500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: cyclin-dependent kinase inhibitor 1B(p27, Kip1)
• Target: CDKN1B
• Molecular Weight: 21 kDa
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: liquid
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27, P27, KIP1, P27KIP1

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with orb395397 (P27; KIP1 Antibody) at dilution of 1:600.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using orb395397 (P27; KIP1 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

IP Result of anti-P27; KIP1 (IP: orb395397, 4 ug; Detection: orb395397 1:300) with NIH/3T3 cells lysate 1600 ug.

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using orb395397 (P27; KIP1 Antibody) at dilution of 1:200. heat mediated antigen retrieved with Tris-EDTA buffer (pH9).

Quick Database Links

Gene Symbol

CDKN1B

UniProt

P46527