CHD3 Recombinant Rabbit Monoclonal Antibody

SKU: orb1499274

Description

CHD3 Recombinant Rabbit Monoclonal Antibody

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: FC, ICC
• Dilution Range: ICC/IF=1:50-200, Flow-Cyt=1:50-100
• Reactivity: Human
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: BT5E8
• Immunogen: KLH conjugated synthetic peptide derived from human CHD3
• Target: CHD3
• Molecular Weight: 226 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CK820_G0036764; Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH; 2600010P09Rik; Chd7; Prp7; Prp9-1; CHD3_HUMAN; CHD3; CHD-3; ATP-dependent helicase CHD3; Mi-2 autoantigen 240 kDa protein; Zinc finger helicase (hZFH); 3.6.4.-; chromodomain helicase DNA binding protein 3

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: C6, 2: HeLa, Protein loading quantity: 20 µg, Exposure time: 1: 60 s, 2: 30 s, Predicted MW: 227 kDa, Observed MW: 260 kDa.

Tissue: Human breast, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499274.

Quick Database Links

Gene Symbol

CHD3

UniProt

Q12873