c-KROX Antibody

SKU: orb750514

Description

c-KROX antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:2,500 - 1:10,000, IHC: 1.25-2.5 µg/ml, WB: 1:500 - 1:3,000
• Reactivity: Human, Mouse, Rat
• Application Notes:
  This antibody has been tested for use in ELISA, immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80 kDa in size corresponding to cKrox by western blotting in the appropriate cell lysate or extract. Lysates from mouse thymocytes are recommended for use as positive controls.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of mouse cKrox protein.
• Target: c-KROX
• Purity: This antibody is directed against mouse cKrox. The product is delipidated and defibrinated antiserum. A BLAST analysis was used to suggest cross-reactivity with cKrox from mouse, human and rat based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 85 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-cKrox Antibody, ThPok antibody, Kruppel related zinc finger protein hcKrox antibody, T-helper inducing POZ/Kruppel-like factor antibody, ZBTB 15 antibody, ZBTB 7B antibody

Immunohistochemistry of cKrox antibody. Tissue: human small intestine. Fixation: formalin fixed paraffin embedded. Antigen retrieval: user optimized. Primary antibody: orb750514 cKrox at 1:100. Secondary antibody: Peroxidase goat anti-rabbit at 1:10000 for 45 min at RT.

Quick Database Links

Gene Symbol

c-KROX

UniProt

Q64321

RefSeq (Protein):NP_033591.2