CLDN4 Antibody

SKU: orb1239033

Description

CLDN4 Antibody

Research Area

Cancer Biology, Cell Biology

Images & Validation

• Tested Applications: ELISA, IF, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-CLAUDIN4 antibody (orb1239033) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human CLAUDIN4. The immunogen is located within the last 50 amino acids of CLAUDIN4.
• Target: CLDN4
• Molecular Weight: Predicted: 22kDObserved: 22 kD
• Purification: CLAUDIN4 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: CLAUDIN4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CLAUDIN4 Antibody: CPER, CPE-R, CPETR, CPETR1, WBSCR8, hCPE-R, CPER, Claudin-4, Clostridium perfringens enterotoxin receptor

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: CLAUDIN4, orb1239033 (1 µg/mL), CLAUDIN4 (1 µg/mL), and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: CLAUDIN4, orb1239033 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Colon Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: CLAUDIN4, orb1239033 (Lane 1: 1 µg/mL and Lane 2: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Colon Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: CLAUDIN4, orb1239033 (Lane 1: 1 µg/mL and Lane 2: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Quick Database Links

Gene Symbol

CLDN4

UniProt

O14493

RefSeq (Protein):NP_001296