CLDN1 Antibody

SKU: orb1239298

Description

CLDN1 Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-CLDN1 antibody (orb1239298) was raised against a peptide corresponding to 20 amino acids near the carboxy terminus of human CLDN1. The immunogen is located within the last 50 amino acids of CLDN1.
• Target: CLDN1
• Molecular Weight: Predicted: 23kDObserved: 23 kD
• Purification: CLDN1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: CLDN1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CLDN1 Antibody: CLD1, SEMP1, ILVASC, CLD1, UNQ481/PRO944, Claudin-1, Senescence-associated epithelial membrane protein

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: CLDN1, orb1239298 (1 µg/mL), CLDN1 (2 µg/mL), and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human HepG2 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: CLDN1, orb1239298 (A: 1 µg/mL and B: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Tissue Lysates. Loading: 15 µg of lysates per lane. Antibodies: CLDN1, orb1239298 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Tissue Lysates. Loading: 15 µg of lysates per lane. Antibodies: CLDN1, orb1239298 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of CLDN1 in HepG2 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling CLDN1 with orb1239298 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Quick Database Links

Gene Symbol

CLDN1

UniProt

O95832

RefSeq:CAG33419