CLEC9A Antibody / DNGR-1

SKU: orb640076

Description

This mAb recognizes mouse DNGR-1, which is also known as CLEC9A, which is a highly specific marker of the CD8a+ and the CD103+ DC subsets, as well as a receptor able to recognize a preformed signal exposed on necrotic cells. In peripheral blood, it is highly restricted on the surface of BDCA31(+) dendritic cells and on a small subset of CD14(+) and CD16(-) monocytes.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FACS, IF, WB
• Dilution Range: Flow cytometry: 1-2ug/10^6 cells in 0.1ml,Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml
• Reactivity: Mouse
• Application Notes:
  Optimal dilution of the CLEC9A antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rat
• Clonality: Monoclonal
• Isotype: Rat IgG1, kappa
• Clone No.: 1F6
• Immunogen: RBL-2H3 cells expressing mouse CLEC9A fused to an HA epitope.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE analysis of purified, BSA-free CLEC9A antibody (clone 1F6) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q8RU4