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Description
Research Area
Images & Validation
−| Tested Applications | FC, ICC, IHC-Fr, IHC-P, IP, WB |
|---|---|
| Dilution Range | WB: 1/1000 IHC: 1/50-1/200 IF: 1/100-1/200 IP: 1/20 FC: 1/100 |
| Reactivity | Goat, Hamster, Human, Monkey, Mouse, Rat |
Key Properties
−| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone No. | B6F33XD5XG0 |
| Immunogen | A synthetic peptide corresponding to carboxyl terminal residues of human COX IV |
| Target | COX4I1 |
| Molecular Weight | Calculated MW: 20 kDa; Observed MW: 17 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
| Concentration | 2 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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WB analysis of orb1566580.

Western blot analysis of COX IV in mouse skeletal muscel and Jurkat lysates using COX IV antibody.

Immunocytochemistry analysis of COX IV in HeLa cells using COX IV antibody.

Immunoprecipitation analysis of COX IV (4D11) in Hela lysates using COX IV antibody.

Immunohistochemistry analysis of paraffin-embedded Human colorectal carcinoma using COX IV antibody (4D11B3E8).High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Flow Cytometry analysis of K562 stained with COX4 (red). Blue line histogram represents the isotype control, normal mouse IgG.
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Protocol Information
COX4I1 Antibody (orb1566580)
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